eSOL(Solubility database of all E.coli proteins) is a database on the solubility of entire ensemble E.coli proteins (Niwa T, Ying BW, Saito K, Jin W, Takada S, Ueda T and Taguchi H, 2009) individually synthesized by PURE system that is chaperon free.

Abstract

eSOL is a database on the solubility of entire ensemble E.coli proteins (Niwa T, Ying BW, Saito K, Jin W, Takada S, Ueda T and Taguchi H, 2009) individually synthesized by PURE system that is chaperon free.
The database is a collaborative product of a protein production team and the information platform in the Targeted Proteins Research Project.

It is to be noted that the experiment was based on ASKA library (A complete Set of E.coli K-12 ORF Archive)constructed by Professor Hirotada Mori at Graduate School of Biological Sciences, Nara Institute of Science and Technology, Japan.

fig.1 ASKA library

Reference

Experimental method and results

Bimodal protein solubility distribution revealed by an aggregation analysis of the entire ensemble of Escherichia coli proteins.
Niwa T, Ying BW, Saito K, Jin W, Takada S, Ueda T, Taguchi H
Proc Natl Acad Sci U.S.A. 2009 Mar 17;106(11):4201-6

ASKA library

Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E.coli K-12 ORF archive): unique resources for biological research.
Kitagawa M, Ara T, Arifuzzaman M, loka-Nakamichi T, Inamoto E, Toyonaga H, Mori H
DNA Res. 2005;12(5):291-9

PURE system

Cell-free translation reconstituted with purified components.
Shimizu Y, Inoue A, Tomari Y, Suzuki T, Yokogawa T, Nishikawa K, Ueda T
Nat Biotechnol. 2001 Aug;19(8):751-5